Anti-Somatostatin Receptor 1 antibody (ab2366)

Overview

  • Product nameAnti-Somatostatin Receptor 1 antibody
    See all Somatostatin Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Somatostatin Receptor 1
  • SpecificityThis antibody reacts with a 60 kD protein, known as Somatostatin Receptor 1.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Rat)A synthetic peptide derived from the C-terminus of rat somatostatin receptor 1

  • Positive control
    • Pancreas
  • General notes


    The Somatostatin family of receptors have five members, sst1-sst5, as well as two isoforms of Somatostatin receptor 2, sst2A and sst2B. They are coupled to G protein and are involved in regulation of numerous processes including secretion of insulin, glucagons and growth hormone and excitation of neurons in central and peripheral nervous system.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThe Somatostatin family of receptors have five members, sst1-sst5, as well as two isoforms of Somatostatin receptor 2, sst2A and sst2B. They are coupled to G protein and are involved in regulation of numerous processes including secretion of insulin, glucagons and growth hormone and excitation of neurons in central and peripheral nervous system.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/75.
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
ICC Use at an assay dependent concentration.

Target

  • FunctionReceptor for somatostatin with higher affinity for somatostatin-14 than -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and Na(+)/H(+) exchanger via pertussis toxin insensitive G proteins.
  • Tissue specificityFetal kidney, fetal liver, and adult pancreas, brain, lung, jejunum and stomach.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G-protein coupled receptor antibody
    • Somatostatin R1 antibody
    • Somatostatin receptor 1 antibody
    • Somatostatin receptor isoform 1 antibody
    • Somatostatin receptor type 1 antibody
    • SRIF 2 antibody
    • SRIF-2 antibody
    • SRIF2 antibody
    • SS-1-R antibody
    • SS1 R antibody
    • SS1-R antibody
    • SS1R antibody
    • SSR1_HUMAN antibody
    • SSTR 1 antibody
    • Sstr1 antibody
    see all

Anti-Somatostatin Receptor 1 antibody images

  • Formalin fixed paraffin embedded human pancreas stained with Somatostatin Receptor using ABC and AEC chromogen.
  • All lanes : Anti-Somatostatin Receptor 1 antibody (ab2366) at 1 µg/ml

    Lane 1 : PANC-1 nuclear extract lysate (ab14882)
    Lane 2 : Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab2366 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2366, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Somatostatin Receptor 1 antibody (ab2366)

This product has been referenced in:
  • Fasciani A  et al. Overexpression and functional relevance of somatostatin receptor-1, -2, and -5 in endometrium and endometriotic lesions. J Clin Endocrinol Metab 95:5315-9 (2010). IHC-P ; Human . Read more (PubMed: 20739383) »

See 1 Publication for this product

Product Wall

This antibody is directed against somatostatin receptor 1.

Gracias por contactarnos.

La concentración de Ig de este anticuerpo es de 66.6ug/ml. Las diluciones óptimas varían en el rango entre 1:40 y 1:400.

Si necesitas consultar nuestros protocolos online, te adjunto el...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
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Abcam user community

Verified customer

Submitted Jun 29 2011

I'm sorry to hear you are experiencing problems with ab2366 in immunocytochemistry. This antibody has not been tested in this application, therefore I am unable to provide a specific protocol for this staining method, but I after consultation with ...

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Thank you for your enquiry. This antibody was produced in-house. The paper by Schulz listed on the data sheet, is only a general reference concerning the receptor. If you require further information, please contact us again and we will gladly assist you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"