Anti-Somatostatin Receptor 2 antibody (ab124144)

Overview

  • Product name
    Anti-Somatostatin Receptor 2 antibody
    See all Somatostatin Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Somatostatin Receptor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chinese hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Somatostatin Receptor 2.

  • Positive control
    • This antibody gave a positive signal in Rat and Mouse Pancreas tissue lysates within WB and in Mouse Pancreas formalin fixed paraffin embedded tissue section within IHC-P.

Properties

Applications

Our Abpromise guarantee covers the use of ab124144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).

Target

  • Function
    Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
  • Tissue specificity
    Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Somatostatin receptor type 2 antibody
    • SRIF-1 antibody
    • SRIF1 antibody
    • SS-2-R antibody
    • SS2-R antibody
    • SS2R antibody
    • SSR2_HUMAN antibody
    • SST2 antibody
    • SSTR2 antibody
    see all

Images

  • All lanes : Anti-Somatostatin Receptor 2 antibody (ab124144) at 1 µg/ml

    Lane 1 : Pancreas (Rat) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 27 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 6 minutes


    Somatostatin Receptor 2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124144 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • IHC image of Somatostatin Receptor 2 staining in mouse normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124144, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab124144 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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