Recombinant Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
See all Somatostatin Receptor 2 primary antibodies -
Description
Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal -
Host species
Rabbit -
Specificity
The specificity of this antibody was demonstrated in a Somatostatin Receptor 2-deficient mouse model. Refer to Fischer et al.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab171899) -
Positive control
- WB: Human and mouse brain tissue lysate. U937, INS-1 and Somatostatin Receptor 2 transfected HEK-293 cell lysates. IHC-P: Human pancreas, brain and breast carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59.49% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
UMB1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 75-80 kDa (predicted molecular weight: 41 kDa).Can be blocked with Somatostatin Receptor 2 peptide (ab171899).
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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IHC-P |
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 75-80 kDa (predicted molecular weight: 41 kDa).Can be blocked with Somatostatin Receptor 2 peptide (ab171899). This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
IHC-P
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels. -
Tissue specificity
Cerebrum and kidney. In lesser amounts in jejunum, colon and liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6752 Human
- Entrez Gene: 20606 Mouse
- Entrez Gene: 54305 Rat
- Omim: 182452 Human
- SwissProt: P30874 Human
- SwissProt: P30875 Mouse
- SwissProt: P30680 Rat
- Unigene: 514451 Human
see all -
Alternative names
- Somatostatin receptor type 2 antibody
- SRIF-1 antibody
- SRIF1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas labelling somatostatin receptor 2 with ab134152 at a concentration of 0.2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab134152 anti somatostatin receptor 2 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
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Tissue Microarrays stained for Anti-Somatostatin Receptor 2 antibody [UMB1] using ab134152 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested.The sections were incubated with ab134152 for 30 mins at room temperature used at 1:2000 dilution (0.928 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Counterstain was Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Somatostatin Receptor 2 with ab134152 at 1/2000 dilution (0.928 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with ab134152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution
Lane 1 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in RIPA lysis method
Lane 2 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in 1% SDS hot lysis method
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
Lane 7 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
This antibody doesn’t work in MCF-7 and MDA-MB-231 cells which should be positive (PMID: 16519802). A549 cell is Somatostatin Receptor 2 negative cell line (PMID: 19148511). -
Immunohistochemical analysis of paraffin-embedded human cervical cancer xenograft tissue (cells derived from liver metastases) using ab134152 at 1/100 dilution.
Somatostatin Receptor 2 is detected using ab134152.
From Figure 1 of Yuan et al.
Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617
Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/
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All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1% SDS hot lysis method
Lane 3 : Mouse brain lysates prepared in RIPA lysis method
Lane 4 : Mouse brain lysates prepared in 1% SDS hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 and 2: 1 second.
Lane 3 and 4: 5 seconds. -
Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution
Lane 1 : U937 (Human histiocytic lymphoma cell line) cell lysates
Lane 2 : INS-1 cell lysates
Lane 3 : Somatostatin Receptor 2 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (111)
ab134152 has been referenced in 111 publications.
- Elvborn M et al. Hyperfractionated Treatment with 177Lu-Octreotate Increases Tumor Response in Human Small-Intestine Neuroendocrine GOT1 Tumor Model. Cancers (Basel) 14:N/A (2022). PubMed: 35008397
- Ilie MD et al. Intratumoural spatial distribution of S100B + folliculostellate cells is associated with proliferation and expression of FSH and ERα in gonadotroph tumours. Acta Neuropathol Commun 10:18 (2022). PubMed: 35139928
- Watanabe H et al. Somatostatin Receptor 2 Expression Profiles and Their Correlation with the Efficacy of Somatostatin Analogues in Gastrointestinal Neuroendocrine Tumors. Cancers (Basel) 14:N/A (2022). PubMed: 35159042
- Forest F et al. High-grade childhood intra-parenchymal brain tumor clustering with ATRT and expanding the cancer spectrum related to inherited SMARCE1 truncating variations. Acta Neuropathol Commun 10:24 (2022). PubMed: 35164878
- Wang AZ et al. Single-cell profiling of human dura and meningioma reveals cellular meningeal landscape and insights into meningioma immune response. Genome Med 14:49 (2022). PubMed: 35534852