Anti-Somatostatin Receptor 4 antibody (ab49298)

Overview

  • Product nameAnti-Somatostatin Receptor 4 antibody
    See all Somatostatin Receptor 4 primary antibodies
  • Description
    Rabbit polyclonal to Somatostatin Receptor 4
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    A region within synthetic peptide: IAIFADTRPA RGGQAVACNL QWPHPAWSAV FVVYTFLLGF LLPVLAIGLC, corresponding to internal sequence amino acids 181-230 of Human Somatostatin Receptor 4

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

WB Use a concentration of 1.25 mg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionReceptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.
  • Tissue specificitySpecifically expressed in fetal and adult brain, lung tissue, stomach, and in lesser quantities in the kidney, pituitary and adrenals.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Somatostatin receptor type 4 antibody
    • SS 4R antibody
    • SS-4-R antibody
    • SS4-R antibody
    • SS4R antibody
    • SSR4_HUMAN antibody
    • SST R4 antibody
    • SSTR 4 antibody
    • Sstr4 antibody
    see all

Anti-Somatostatin Receptor 4 antibody images

  • Lane 1 :
    Lane 2 : Anti-Somatostatin Receptor 4 antibody (ab49298) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ICC/IF image of ab49298 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Somatostatin Receptor 4 antibody (ab49298)

ab49298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"