Recombinant
RabMAb

Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

Overview

  • Product name
    Anti-Somatostatin Receptor 5 antibody [UMB4]
    See all Somatostatin Receptor 5 primary antibodies
  • Description
    Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Somatostatin Receptor 5 aa 300-400 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab178801)

  • Positive control
    • Human fetal heart and HT29 cell lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Panc-1
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.Can be blocked with Somatostatin Receptor 5 peptide (ab178801).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
    • Tissue specificity
      Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • SSTR5 antibody
      • Somatostatin receptor subtype 5 antibody
      • Somatostatin receptor type 5 antibody
      • SS 5R antibody
      • SS-5-R antibody
      • SS5 R antibody
      • SS5-R antibody
      • SS5R antibody
      • SSR5_HUMAN antibody
      • SST R5 antibody
      • SSTR 5 antibody
      • Sstr5 antibody
      see all

    Images

    • ICC/IF image of ab109495 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109495 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    References

    This product has been referenced in:
    • Venegas-Moreno E  et al. Association between dopamine and somatostatin receptor expression and pharmacological response to somatostatin analogues in acromegaly. J Cell Mol Med 22:1640-1649 (2018). IHC ; Human . Read more (PubMed: 29266696) »
    • Kaemmerer D  et al. Somatostatin and CXCR4 chemokine receptor expression in hepatocellular and cholangiocellular carcinomas: tumor capillaries as promising targets. BMC Cancer 17:896 (2017). IHC-P ; Human . Read more (PubMed: 29282035) »

    See all 14 Publications for this product

    Customer reviews and Q&As


    J’ai contacté le laboratoire qui a accepté d’ajouter le peptide bloquant d’ab109495 au catalogue sous la référence ab178801.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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