This antibody gave a positive signal in Human Thymus tissue lysate as well as U937 and THP1 human whole cell lysates.
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Can be used with methanol or paraformaldehyde-fixed cells.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 263 kDa (predicted molecular weight: 263 kDa).
Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing.
Widely expressed, with the higher expression seen in leukocyte and heart.
Bax antagonist selected in saccharomyces 1 antibody
Negative regulatory element-binding protein antibody
NRE-binding protein antibody
Protein DBP-5 antibody
Protein SON antibody
SON protein antibody
Anti-SON antibody images
Immunocytochemistry/ Immunofluorescence - Anti-SON antibody (ab109472)Image Courtesy of an abreview submitted by Dr. Kirk Mcmanus, Institute of Cancer Care, Univ. of Manitoba
ab109472(1/200) staining SON in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.05% TritonX100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
All lanes : Anti-SON antibody (ab109472) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146) Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 263 kDa Observed band size : 263 kDa Additional bands at : 100 kDa,270 kDa (possible post-translational modification),40 kDa,50 kDa,68 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109472 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
IHC image of SON staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109472, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.