Purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).
Our Abpromise guarantee covers the use of ab50515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/250. PubMed: 18777197|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.|
|IHC-Fr||Use a concentration of 25 µg/ml.|
|Neutralising||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 4 µg/ml.|
|ICC/IF||Use a concentration of 25 µg/ml.|
ab50515 staining Sonic Hedgehog in human Panc-1 cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was used at 25 μg/ml and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 donkey polyclonal to rat Ig, diluted 1/700, was used as the secondary antibody.