Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Sonic Hedgehog.
(Peptide available as ab21448.)
Our Abpromise guarantee covers the use of ab19897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [OTI10H6] to Sonic Hedgehog (ab87382). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 48 kDa).|