Overview

  • Product nameAnti-Sonic Hedgehog antibody
    See all Sonic Hedgehog primary antibodies
  • Description
    Rabbit polyclonal to Sonic Hedgehog
  • Tested applicationsSuitable for: ICC/IF, Sandwich ELISA, WBmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Human, Xenopus laevis, Zebrafish, a wide range of other species
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Sonic Hedgehog.

    (Peptide available as ab21448.)

  • Positive control
    • This antibody gave a positive signal in Mouse Recombinant Sonic Hedgehog protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab19897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 48 kDa).
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionBinds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
    • Tissue specificityExpressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
    • Involvement in diseaseDefects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
      Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
      Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
      Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
    • Sequence similaritiesBelongs to the hedgehog family.
    • Post-translational
      modifications
      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
      Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
      N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
    • Cellular localizationCell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
    • Information by UniProt
    • Database links
    • Alternative names
      • HHG 1 antibody
      • HHG-1 antibody
      • HHG1 antibody
      • HLP 3 antibody
      • HLP3 antibody
      • Holoprosencephaly 3 antibody
      • HPE 3 antibody
      • HPE3 antibody
      • MCOPCB5 antibody
      • shh antibody
      • SHH_HUMAN antibody
      • SMMC I antibody
      • SMMCI antibody
      • Sonic Hedgehog (Drosophila) homolog antibody
      • sonic hedgehog homolog (Drosophila) antibody
      • Sonic hedgehog homolog antibody
      • Sonic hedgehog protein antibody
      • Sonic hedgehog protein C-product antibody
      • TPT antibody
      • TPTPS antibody
      see all

    Anti-Sonic Hedgehog antibody images

    Protocols

    References for Anti-Sonic Hedgehog antibody (ab19897)

    This product has been referenced in:
    • Zerr P  et al. Inhibition of hedgehog signaling for the treatment of murine sclerodermatous chronic graft-versus-host disease. Blood 120:2909-17 (2012). Human, Mouse . Read more (PubMed: 22915638) »
    • Hasebe T  et al. Epithelial-Connective Tissue Interactions Induced by Thyroid Hormone Receptor Are Essential for Adult Stem Cell Development in the Xenopus laevis Intestine. Stem Cells 29:154-61 (2011). IHC-P ; Xenopus laevis . Read more (PubMed: 21280164) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry free floating
    Sample Zebrafish Tissue sections (Spinal Cord)
    Specification Spinal Cord
    Username

    Abcam user community

    Verified customer

    Submitted Mar 01 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Leucoraja erinacea Embryo (Whole embryo)
    Specification Whole embryo
    Username

    Dr. Andrew Gillis

    Verified customer

    Submitted Jul 25 2013

    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2013

    Thank you for your call today, and I apologize that these antibodies still are not working.

    As we discussed, I'm sending a free of charge ELISA kit ab100639 on the order ***, which I expect to arrive by Friday.

    Please keep me update...

    Read More

    Thank you for your call last week and for your patience while I have been in touch with my colleagues about these antibodies. I apologize for the delay getting back to you.

    The capture antibody ab87382 was tested at a coating concentration of...

    Read More

    DISCOUNT CODE: XXXXXXXX
    Expiration date: 18-05-2012

    I am very pleased to hear you would like to accept our offer and test ab19897 in ZebraFish. This code will give you 1 free [PRIMARY ANTIBODY/PROTEIN] before the expiration date. To red...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Intact brain)
    Specification Intact brain
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Dr. Ruxandra Sirbulescu

    Verified customer

    Submitted Sep 28 2011

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human (anal cancer), mouse (adenoma), rat (thymus))
    Specification Human (anal cancer), mouse (adenoma), rat (thymus)
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization No
    Blocking step sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 09 2008

    Application Western blot
    Sample Human Cell lysate - whole cell (IMR-32, F9, MEF, PC12)
    Loading amount 20 µg
    Specification IMR-32, F9, MEF, PC12
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 03 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (e14.5 brain)
    Specification e14.5 brain
    Fixative Paraformaldehyde
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Dr. Dean Griffiths

    Verified customer

    Submitted Jan 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"