A synthetic peptide corresponding to N-terminus of human Sonic Hedgehog
Our Abpromise guarantee covers the use of ab73958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Human lung tissue stained with ab73958 at 1/100 dilution for 30 mins at RT.
Tissue sections boiled in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.