The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged Sorbitol Dehydrogenase is approximately 0.1ng/ml as a capture antibody.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionConverts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
Tissue specificityExpressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
Cellular localizationMitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.