Anti-Sorbitol Dehydrogenase antibody (ab66065)

Overview

  • Product nameAnti-Sorbitol Dehydrogenase antibody
    See all Sorbitol Dehydrogenase primary antibodies
  • Description
    Mouse monoclonal to Sorbitol Dehydrogenase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Orangutan
  • Immunogen

    Recombinant tagged fragment: MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR, corresponding to amino acids 1-111 of Human Sorbitol Dehydrogenase

  • Positive control
    • Hela nuclear extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged Sorbitol Dehydrogenase is approximately 0.1ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionConverts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
    • Tissue specificityExpressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
    • Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
    • Cellular localizationMitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • DHSO_HUMAN antibody
      • L iditol 2 dehydrogenase antibody
      • L-iditol 2-dehydrogenase antibody
      • OTTHUMP00000161939 antibody
      • SDH antibody
      • Sorbitol dehydrogenase 1 antibody
      • Sorbitol dehydrogenase antibody
      • SORD 1 antibody
      • SORD antibody
      • SORD1 antibody
      see all

    Anti-Sorbitol Dehydrogenase antibody images

    • Anti-Sorbitol Dehydrogenase antibody (ab66065) at 1 µg/ml + HeLa nuclear extract at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    References for Anti-Sorbitol Dehydrogenase antibody (ab66065)

    ab66065 has not yet been referenced specifically in any publications.

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