Anti-Sorbitol Dehydrogenase antibody (ab88509)

Overview

  • Product nameAnti-Sorbitol Dehydrogenase antibody
    See all Sorbitol Dehydrogenase primary antibodies
  • Description
    Mouse polyclonal to Sorbitol Dehydrogenase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human protein (NP_003095.1)

  • Positive control
    • Human liver or Jurkat cell lysate. Human prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab88509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionConverts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
    • Tissue specificityExpressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
    • Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
    • Cellular localizationMitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • DHSO_HUMAN antibody
      • L iditol 2 dehydrogenase antibody
      • L-iditol 2-dehydrogenase antibody
      • OTTHUMP00000161939 antibody
      • SDH antibody
      • Sorbitol dehydrogenase 1 antibody
      • Sorbitol dehydrogenase antibody
      • SORD 1 antibody
      • SORD antibody
      • SORD1 antibody
      see all

    Anti-Sorbitol Dehydrogenase antibody images

    • Anti-Sorbitol Dehydrogenase antibody (ab88509) at 1 µg/ml + Human liver lysate at 50 µg/ml

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-Sorbitol Dehydrogenase antibody (ab88509) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Sorbitol Dehydrogenase antibody (ab88509) at 1 µg/ml

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Purified ab88509 at 3ug/ml staining Sorbitol Dehydrogenase in formalin-fixed, paraffin-embedded Human prostate tissue.

    References for Anti-Sorbitol Dehydrogenase antibody (ab88509)

    ab88509 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Wild-type Prostate)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Permeabilization No
    Specification Wild-type Prostate
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative Paraformaldehyde
    Username

    Dr. Lucy Penfold

    Verified customer

    Submitted Nov 09 2016

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacemen...

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    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacemen...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"