Overview

  • Product name
    Anti-SorCS1 antibody
  • Description
    Rabbit polyclonal to SorCS1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N terminal region of Human SorCS1 (NP_443150.3).

  • Positive control
    • K562 cell line lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab93331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 130 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      SorCS1 belongs to the family of VPS10 domain-containing receptors (the name is derived from the yeast vacuolar protein sorting protein-10, which is involved in sorting of carboxy-peptidase Y from the Golgi apparatus to the vacuole). SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.
    • Cellular localization
      Membrane; Single-pass type I membrane protein
    • Database links
    • Alternative names
      • FLJ41758 antibody
      • FLJ43475 antibody
      • FLJ44957 antibody
      • hSorCS antibody
      • OTTHUMP00000058855 antibody
      • SorCS 1 antibody
      • SORCS antibody
      • SORCS receptor 1 antibody
      • Sortilin related VPS10 domain containing receptor 1 antibody
      • VPS10 domain containing receptor SorCS1 antibody
      • VPS10 domain receptor protein SORCS 1 antibody
      • VPS10 domain receptor protein SORCS antibody
      • VPS10 domain receptor protein SORCS1 antibody
      • VPS10 domain receptor SorCS antibody
      see all

    Images

    • ICC/IF image of ab93331 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93331 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-SorCS1 antibody (ab93331) at 1/1000 dilution + K562 whole cell lysate at 35 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

      Predicted band size : 130 kDa

      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    ab93331 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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