Overview

  • Product nameAnti-SorLA antibody
    See all SorLA primary antibodies
  • Description
    Rabbit polyclonal to SorLA
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2150 to the C-terminus of Human SorLA.

    (Peptide available as ab16691.)

  • Positive control
    • rat brain tissue, most abundant expression has been identified in the cerebellum, cerebral cortex and the occipital pole.

Properties

Applications

Our Abpromise guarantee covers the use of ab16642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IHC (PFA fixed) Use a concentration of 0.6 - 1 µg/ml.

Target

  • FunctionLikely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction.
  • Tissue specificityExpressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals.
  • Sequence similaritiesBelongs to the SORL1 family.
    Contains 5 BNR repeats.
    Contains 1 EGF-like domain.
    Contains 6 fibronectin type-III domains.
    Contains 11 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.
  • Post-translational
    modifications
    The propeptide removed in the N-terminus may be cleaved by furin or homologous proteases.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf32 antibody
    • FLJ21930 antibody
    • FLJ39258 antibody
    • gp250 antibody
    • LDLR relative with 11 ligand binding repeats antibody
    • LDLR relative with 11 ligand-binding repeats antibody
    • Low density lipoprotein receptor relative with 11 ligand binding repeats antibody
    • Low-density lipoprotein receptor relative with 11 ligand-binding repeats antibody
    • LR 11 antibody
    • LR11 antibody
    • LRP 9 antibody
    • LRP9 antibody
    • Mosaic protein LR11 antibody
    • SORL 1 antibody
    • SORL_HUMAN antibody
    • SORL1 antibody
    • SorLA 1 antibody
    • SorLA antibody
    • SorLA-1 antibody
    • Sortilin related receptor antibody
    • Sortilin related receptor L(DLR class) A repeats containing antibody
    • Sortilin-related receptor antibody
    • Sorting protein related receptor containing LDLR class A repeats antibody
    • Sorting protein-related receptor containing LDLR class A repeats antibody
    see all

Anti-SorLA antibody images

  • Immunofluorescent staining for SorLA in rat cortical neurons using Rabbit polyclonal to SorLA (ab16642). The staining is cytoplasmic and punctate in these cortical neurons.

    Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab16642 was incubated overnight at 1/500 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

  • Image courtesy of Human Protein Atlas
    ab16642 staining SorLA in paraffin embedded human cerebellum tissues, showing a strong signal in the granular and molecular cell layers and Purkinje cells. Paraffin embedded human cerebellum sections (4µm) were incubated with ab16642 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16642 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

References for Anti-SorLA antibody (ab16642)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Cortex)
Loading amount 30 µg
Specification Cortex
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Balmiki Ray

Verified customer

Submitted Jun 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (sections of rat brain)
Specification sections of rat brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Dec 21 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"