The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 246 kDa.
Peptide ELISA: antibody detection limit dilution 1:4,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLikely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction.
Tissue specificityExpressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals.
Sequence similaritiesBelongs to the SORL1 family. Contains 5 BNR repeats. Contains 1 EGF-like domain. Contains 6 fibronectin type-III domains. Contains 11 LDL-receptor class A domains. Contains 5 LDL-receptor class B repeats.
Post-translational modificationsThe propeptide removed in the N-terminus may be cleaved by furin or homologous proteases.