Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab16640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 19761405ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration. PubMed: 21357693
ICC 1/1000.
IHC-FoFr Use a concentration of 0.2 - 0.5 µg/ml.

Target

  • FunctionFunctions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
  • Tissue specificityExpressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
  • Involvement in diseaseNote=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction.
  • Sequence similaritiesBelongs to the VPS10-related sortilin family. SORT1 subfamily.
    Contains 9 BNR repeats.
  • DomainThe N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
    The extracellular domain may be shed following protease cleavage in some cell types.
  • Post-translational
    modifications
    The N-terminal propeptide is cleaved by furin and possibly other homologous proteases.
  • Cellular localizationMembrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa NT receptor antibody
    • Glycoprotein 95 antibody
    • Gp 95 antibody
    • Gp95 antibody
    • LDLCQ6 antibody
    • Neurotensin receptor 3 antibody
    • NT 3 antibody
    • NT3 antibody
    • NTR 3 antibody
    • NTR3 antibody
    • OTTHUMP00000013784 antibody
    • SORT 1 antibody
    • SORT_HUMAN antibody
    • SORT1 (gene name) antibody
    • Sort1 antibody
    • Sortilin 1 antibody
    • Sortilin antibody
    see all

Anti-Sortilin antibody images



  • Predicted band size : 95 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Sortilin knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: 293T cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab16440 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab16640 was shown to specifically react with Sortilin when Sortilin knockout samples were used. Wild-type and Sortilin knockout samples were subjected to SDS-PAGE. ab16640 and ab8245 (loading control to GAPDH) were diluted 1 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776
    secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Immunofluorescent staining for Sortilin in rat cortical neurons using Rabbit polyclonal to Sortilin (ab16640). The staining is cytoplasmic and punctate of many cells such as cortical neurons. The image was taken with a X20 objective.

    Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT and then cut on cryostat (30µm coronal sections). IHC was perfored in free floating with fixed tissues (rat brain sections). Primary antibody was incubated overnight at 1/3000 at room temperature

  • ab16640 staining cultured mouse astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab16640 at 1/1000 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Sortilin expressed in the cytosol mainly in trans-golgi compartments.

    See Abreview

  • Lane 1 : Marker
    Lanes 2 - 4 : Anti-Sortilin antibody (ab16640) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Human Brain Tissue Lysate at 20 µg
    Lane 3 : Mouse Brain at 20 µg
    Lane 4 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg

    Secondary
    Lanes 2 - 4 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution


    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Additional bands at : 31 kDa (possible cleavage fragment).
  • ICC/IF image of ab16640 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Sortilin antibody (ab16640)

This product has been referenced in:
  • Ezkurdia N  et al. Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats. PLoS One 9:e84374 (2014). WB . Read more (PubMed: 24400086) »
  • Campagnolo L  et al. Sortilin expression is essential for pro-nerve growth factor-induced apoptosis of rat vascular smooth muscle cells. PLoS One 9:e84969 (2014). IF, ICC . Read more (PubMed: 24404198) »

See all 18 Publications for this product

Product Wall

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Abreviews
Application Western blot
Sample Rat Tissue lysate - whole (Rat brain-Hippocampus, cortex)
Loading amount 20 µg
Specification Rat brain-Hippocampus, cortex
Gel Running Conditions Non-reduced Denaturing (4-12 % Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Astrocytes culture)
Specification Astrocytes culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cultured Cells (Hippocampal neurons)
Specification Hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Loading amount 40 µg
Specification Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Youngjeon Lee

Verified customer

Submitted Dec 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Schwann Cells)
Loading amount 45 µg
Specification Schwann Cells
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
Username

Matthew Provenzano

Verified customer

Submitted Mar 27 2007

Thank you for getting back to me and for sharing your recent findings with me. I am very sorry to hear that even after following my suggestions, you are still not satisfied with this antibody. This product has been tested for IHC by one of our coll...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Rat brain)
Specification Rat brain
Fixative Paraformaldehyde
Antigen retrieval step None
Username

Dr. Sophie Pezet

Verified customer

Submitted Nov 02 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"