Overview

  • Product name
    Anti-Sortilin antibody [EPR15010]
    See all Sortilin primary antibodies
  • Description
    Rabbit monoclonal [EPR15010] to Sortilin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Sortilin aa 650-800. The exact sequence is proprietary.
    Database link: Q99523

  • Positive control
    • WB: SW40, HeLa and SH-SY5Y cell lysates. IHC-P: Human brain tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.

Perform heat mediated antigen retrieval with EDTA Buffer, pH9 before commencing with IHC protocol.

WB 1/1000 - 1/2000. Detects a band of approximately 92-114 kDa (predicted molecular weight: 92 kDa).

Target

  • Function
    Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
  • Tissue specificity
    Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
  • Involvement in disease
    Note=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction.
  • Sequence similarities
    Belongs to the VPS10-related sortilin family. SORT1 subfamily.
    Contains 9 BNR repeats.
  • Domain
    The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
    The extracellular domain may be shed following protease cleavage in some cell types.
  • Post-translational
    modifications
    The N-terminal propeptide is cleaved by furin and possibly other homologous proteases.
  • Cellular localization
    Membrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa NT receptor antibody
    • Glycoprotein 95 antibody
    • Gp 95 antibody
    • Gp95 antibody
    • LDLCQ6 antibody
    • Neurotensin receptor 3 antibody
    • NT 3 antibody
    • NT3 antibody
    • NTR 3 antibody
    • NTR3 antibody
    • OTTHUMP00000013784 antibody
    • SORT 1 antibody
    • SORT_HUMAN antibody
    • SORT1 (gene name) antibody
    • Sort1 antibody
    • Sortilin 1 antibody
    • Sortilin antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Sortilin knockout HAP1 cell lysate (20 µg)
    Lane 3: SW480 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab188586 observed at 92 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab188586 was shown to  react with Sortilin in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Sortilin knockout samples were examined. Wild-type and Sortilin knockout samples were subjected to SDS-PAGE. ab188586 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sortilin with ab188586 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-Sortilin antibody [EPR15010] (ab188586) at 1/1000 dilution

    Lane 1 : SH-SY5Y whole cell lysate
    Lane 2 : Human hippocampus tissue lysate
    Lane 3 : Human fetal brain tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : 3T3-L1 whole cell lysate
    Lane 6 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 92 kDa
    Observed band size: 92 kDa


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Sortilin antibody [EPR15010] (ab188586) at 1/2000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 92 kDa

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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