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Overview

  • Product name
    Anti-SOS1 antibody
    See all SOS1 primary antibodies
  • Description
    Rabbit polyclonal to SOS1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SOS1.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab35701.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HEK293 Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) IHC-P (FFPE): Hu Placenta (normal) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab35702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Promotes the exchange of Ras-bound GDP by GTP.
  • Tissue specificity
    Expressed in gingival tissues.
  • Involvement in disease
    Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
    Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 PH domain.
    Contains 1 Ras-GEF domain.
  • Information by UniProt
  • Database links
    • Alternative names
      • alternate SOS1 antibody
      • GF1 antibody
      • GGF1 antibody
      • GINGF antibody
      • gingival fibromatosis antibody
      • gingival fibromatosis hereditary 1 antibody
      • Guanine nucleotide exchange factor antibody
      • HGF antibody
      • NS4 antibody
      • Son of sevenless homolog 1 (Drosophila) antibody
      • Son of sevenless homolog 1 antibody
      • SOS Ras/Rac guanine nucleotide exchange factor 1 antibody
      • SOS-1 antibody
      • Sos1 antibody
      • SOS1_HUMAN antibody
      see all

    Anti-SOS1 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody (ab35702)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody (ab35702)

      IHC image of SOS1 staining in Human Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab35702, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    • Western blot - SOS1 antibody (ab35702)
      Western blot - SOS1 antibody (ab35702)
      All lanes : Anti-SOS1 antibody (ab35702) at 1 µg/ml

      Lane 1 : HEK293 whole cell lysate (ab7902)
      Lane 2 : HepG2 whole cell lysate (ab7900)
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 170 kDa
      Observed band size : 165 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-SOS1 antibody (ab35702)

    ab35702 has not yet been referenced specifically in any publications.

    Publishing research using ab35702? Please let us know so that we can cite the reference in this datasheet.

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