- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-SOS1 antibody
See all SOS1 primary antibodies -
DescriptionRabbit polyclonal to SOS1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SOS1.
(Peptide available as ab35701.)
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HEK293 Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) IHC-P (FFPE): Hu Placenta (normal) tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab35702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionPromotes the exchange of Ras-bound GDP by GTP.
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Tissue specificityExpressed in gingival tissues.
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Involvement in diseaseDefects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common. -
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PH domain.
Contains 1 Ras-GEF domain. - Information by UniProt
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Database links
- Entrez Gene: 6654 Human
- Entrez Gene: 20662 Mouse
- Omim: 182530 Human
- SwissProt: Q07889 Human
- SwissProt: Q62245 Mouse
- Unigene: 709893 Human
- Unigene: 360004 Mouse
- Unigene: 60975 Mouse
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Alternative names
- alternate SOS1 antibody
- GF1 antibody
- GGF1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody (ab35702)IHC image of SOS1 staining in Human Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab35702, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-SOS1 antibody (ab35702)All lanes : Anti-SOS1 antibody (ab35702) at 1 µg/ml
Lane 1 :HEK293 whole cell lysate (ab7902)
Lane 2 :HepG2 whole cell lysate (ab7900)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 170 kDa
Observed band size: 165 kDa (why is the actual band size different from the predicted?)
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.
References
ab35702 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"