All lanes : Anti-SOS2 antibody (ab85831) at 0.1 mg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 50 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of ab85831 Lane 1: ab85831 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab85831 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.
References for Anti-SOS2 antibody (ab85831)
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