Overview

  • Product name
  • Description
    Rabbit polyclonal to SOS2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1282 -1332 of Human SOS2 (NP_008870.2)

  • Positive control
    • HeLa and 293T whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 153 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Promotes the exchange of Ras-bound GDP by GTP.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 PH domain.
    Contains 1 Ras-GEF domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ25596 antibody
    • Guanine nucleotide exchange factor antibody
    • Guanine nucleotide releasing factor antibody
    • Son of sevenless (Drosophilia) homolog 2 antibody
    • Son of sevenless homolog 2 (Drosophila) antibody
    • Son of sevenless homolog 2 antibody
    • SOS 2 antibody
    • SOS-2 antibody
    • Sos2 antibody
    • SOS2_HUMAN antibody
    see all

Images

  • All lanes : Anti-SOS2 antibody (ab85831) at 0.1 mg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 5 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 50 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 153 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Immunoprecipitation/ Western Blot of ab85831
    Lane 1: ab85831 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab85831 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.

References

ab85831 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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