Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab109290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    Anti-SOX1 antibody [EPR4766] images

    • All lanes : Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution

      Lane 1 : NCCIT cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
    • Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue using ab109290 at a dilution of 1/100.
    • Immunofluorescent staining of NCCIT cells using ab109290 at a dilution of 1/100.
    • Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX1 with unpurified ab109290 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    References for Anti-SOX1 antibody [EPR4766] (ab109290)

    This product has been referenced in:
    • Popovic J  et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB ; Human . Read more (PubMed: 24637840) »
    • Ko HA  et al. Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum. Neurosci Lett 548:44-9 (2013). ICC/IF ; Mouse . Read more (PubMed: 23684982) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (ES cells)
    Permeabilization Yes - 0.1% PBST
    Specification ES cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 08 2016

    Application IHC - Wholemount
    Sample Mouse Embryo (E8.5)
    Specification E8.5
    Username

    Abcam user community

    Verified customer

    Submitted Dec 08 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Brain e14)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Brain e14
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 02 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (E10.5 mouse embryo)
    Permeabilization Yes - PBS/0.1% Triton X-100
    Specification E10.5 mouse embryo
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: R.T°C
    Fixative Formaldehyde
    Username

    Dr. Anestis Tsakiridis

    Verified customer

    Submitted Oct 30 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"