Overview

  • Product name
  • Description
    Rabbit polyclonal to SOX15
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: GPLWGPGYAT TQPSRGFGYR PPSYSTAYLP GSYGSSHCKL EAPSPCSLPQ, corresponding to internal sequence amino acids 145-194 of Human SOX15

  • Positive control
    • Transfected 293T cell lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab55960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Binds to the 5'-AACAAT-3' sequence.
  • Tissue specificity
    Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein SOX-12 antibody
    • Protein SOX-15 antibody
    • Protein SOX-20 antibody
    • SOX 12 protein antibody
    • SOX 15 antibody
    • SOX 15 protein antibody
    • SOX 20 protein antibody
    • SOX12 antibody
    • SOX12 protein antibody
    • SOX15 antibody
    • SOX15 protein antibody
    • SOX15_HUMAN antibody
    • SOX20 antibody
    • SOX20 protein antibody
    • SOX26 antibody
    • SOX27 antibody
    • SRY box 15 antibody
    • SRY sex determining region Y box 15 antibody
    • SRY sex determining region Y box 20 antibody
    • SRYbox 15 antibody
    see all

Images

  • Immunohistochemistry of (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SOX15 with ab55960 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubules. Magnification: 400X.

  • Anti-SOX15 antibody (ab55960) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Gel concentration 12%

References

This product has been referenced in:
  • Proestling K  et al. Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients. Reprod Biol Endocrinol 14:81 (2016). IHC-P ; Human . Read more (PubMed: 27881125) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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