Overview

  • Product nameAnti-SOX17 antibody [3B10]
    See all SOX17 primary antibodies
  • Description
    Mouse monoclonal [3B10] to SOX17
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein, expressed in E.coli corresponding to amino acids 177-414 of Human SOX17

  • Positive control
    • WB: HepG2, HeLa, SVT2, A549, COS7, Jurkat and MDCK cell lysates. ICC/IF: HT29 cells. Flow Cyt: Transfected HEK293 expressing SOX17.
  • General notesab84990 should be diluted with PBS before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab84990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/5000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/50 - 1/100.

Target

  • FunctionActs as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells.
  • Tissue specificityExpressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.
  • Involvement in diseaseDefects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3) [MIM:613674]. VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
    Contains 1 Sox C-terminal domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22252 antibody
    • SOX17 antibody
    • SOX17_HUMAN antibody
    • SRY (sex determining region Y) box 17 antibody
    • SRY box 17 antibody
    • SRY related HMG box transcription factor SOX17 antibody
    • Transcription factor SOX-17 antibody
    • Transcription factor SOX17 antibody
    • VUR3 antibody
    see all

Anti-SOX17 antibody [3B10] images

  • Immunocytochemistry/Immunofluorescence analysis of HT29 cells labelling SOX17 with ab84990.

     

  • All lanes : Anti-SOX17 antibody [3B10] (ab84990) at 1/200 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 44 kDa
  • Flow cytometry analysis of HEK293T cells transfected with SOX17 (red) or an empty vector control plasmid (blue) labelling SOX17 with ab84990.

References for Anti-SOX17 antibody [3B10] (ab84990)

This product has been referenced in:
  • Pennarossa G  et al. Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells. Proc Natl Acad Sci U S A 110:8948-53 (2013). Human . Read more (PubMed: 23696663) »
  • Zhang X  et al. Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming. Oncogene : (2012). Read more (PubMed: 22777357) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (seminoma)
Gel Running Conditions Reduced Denaturing (Bolt Bis-Tris 4-12% SDS PAGE)
Loading amount 156000 cells
Specification seminoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (NPCs derived from iPSC)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification NPCs derived from iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (iCell® Neurons)
Permeabilization No
Specification iCell® Neurons
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Acetone
Username

Abcam user community

Verified customer

Submitted Sep 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"