Overview

  • Product name
  • Description
    Rabbit polyclonal to SOX17
  • Host species
    Rabbit
  • Specificity
    Predicted not cross-react with other SOX proteins.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human SOX17 (internal sequence). 16 amino acids.
    Database link: Q9H6I2

  • Positive control
    • HepG2 cell lysate. Mouse liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab191699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells.
  • Tissue specificity
    Expressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.
  • Involvement in disease
    Defects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3) [MIM:613674]. VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
    Contains 1 Sox C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22252 antibody
    • SOX17 antibody
    • SOX17_HUMAN antibody
    • SRY (sex determining region Y) box 17 antibody
    • SRY box 17 antibody
    • SRY related HMG box transcription factor SOX17 antibody
    • Transcription factor SOX-17 antibody
    • Transcription factor SOX17 antibody
    • VUR3 antibody
    see all

Images

  • Lane 1 : Anti-SOX17 antibody (ab191699) at 0.5 µg/ml
    Lane 2 : Anti-SOX17 antibody (ab191699) at 1 µg/ml

    All lanes : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 44 kDa

  • Immunofluorescent analysis of mouse liver tissue labeling SOX17 with ab191699 at 20 μg/ml.

References

This product has been referenced in:
  • Li W  et al. 5-Azacytidine suppresses EC9706 cell proliferation and metastasis by upregulating the expression of SOX17 and CDH1. Int J Mol Med 38:1047-54 (2016). WB ; Human . Read more (PubMed: 27513557) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - nuclear (seminoma)
Gel Running Conditions
Reduced Denaturing (Bolt Bis-Tris 4-12% SDSPAGE)
Loading amount
156000 cells
Specification
seminoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Apr 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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