Overview

  • Product nameAnti-SOX2 antibody [57CT23.3.4]
    See all SOX2 primary antibodies
  • Description
    Mouse monoclonal [57CT23.3.4] to SOX2
  • Tested applicationsICC/IF, IHC - Wholemount, Flow Cyt, Sandwich ELISA, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length SOX2 protein (Human).

  • Positive control
    • 293 cell lysate transfected with SOX2 gene; human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab75485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt 1/10.
Sandwich ELISA Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EPR3131] to SOX2 (ab92494). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Detection.
WB 1/200 - 1/2000. Predicted molecular weight: 34 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody [57CT23.3.4] images

  • Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.
  • Anti-SOX2 antibody [57CT23.3.4] (ab75485) at 1/200 dilution + SOX2 recombinant protein

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SOX2 antibody [57CT23.3.4] (ab75485) at 1/200 dilution

    Lane 1 : non-transfected 293 cell lysate
    Lane 2 : lysate of 293 cells transiently transfected with SOX2 gene

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Human lung carcinoma tissue with ab75485 at 1/50 dilution, using a peroxidase-conjugated secondary antibody, followed by DAB staining.
  • ab75485 staining SOX2 in human schwann cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilised in 0.2% Triton X-100 and then blocked using 10% DCS/ 0.1M lysine/PBS for 30 minutes at 25°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a rabbit anti-mouse IgG conjugated to Alexa Fluor® 568 used at a 1/500 dilution. Cells were counterstained with Hoechst dye to show nuclei.

    See Abreview

  • Immunocytochemistical detection of SOX2 antibody (ab75485) on a mixed rat glial cell culture. Fixative: Paraformaldehyde Permeabilization: 0.3x Triton. Blocking step: 10% serum for 1 hour @ 24°C. Primary antibody used at 1 µg/ml incubated for 24 hours @ 4°C. Secondary antibody: Alexa Fluor® 568 (1/1000). The submitted image shows rat primary glial culture stained with ab75485; DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • ICC/IF image of ab75485 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75485, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing F9 cells stained with ab75485 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75485, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed F9 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Protocols

References for Anti-SOX2 antibody [57CT23.3.4] (ab75485)

This product has been referenced in:
  • Chen S  et al. SOX2 regulates apoptosis through MAP4K4-Survivin signaling pathway in human lung cancer cells. Carcinogenesis 35:613-23 (2014). IHC-P ; Mouse, Human . Read more (PubMed: 24233838) »
  • Peng X  et al. Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice. PLoS One 9:e90707 (2014). ICC/IF ; Mouse . Read more (PubMed: 24595217) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain (tumor))
Specification mouse brain (tumor)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer (pH6)
Permeabilization Yes - PBS + Triton 0.3%
Blocking step I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
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Submitted Dec 21 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cerebellar tumor cells)
Specification cerebellar tumor cells
Fixative Paraformaldehyde
Permeabilization Yes - PBS + Triton 0.3%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Dec 21 2012

DISCOUNT CODE: ABTRIAL-xxxxxx

Ablaufdatum: 13.04.13

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab75485 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutschein...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver)
Specification liver
Fixative Paraformaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver cancer)
Specification liver cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM EDTA
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 12 2012

Thank you for your reply. I am very much in agreement with John that it would be difficult to purify ab92494 to such high concentrations but you should have no troubles in getting the 0.25mg/ml up to the levels you need. You needn't worry about our ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Primary glioblastoma cell line)
Specification Primary glioblastoma cell line
Preparation Cell harvesting/tissue preparation method: Harvest with accutase
Sample buffer: PBS-Tween 0.2%, 2mM EDTA
Fixation ice-cold 70% ethanol
Permeabilization Yes - Tween 0.2%
Gating Strategy Cells incubated with a Mouse IgG
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Submitted Aug 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Primary glioblastoma cell line)
Specification Primary glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 1%
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Human Tissue (Human glioblastoma in mouse brain)
Specification Human glioblastoma in mouse brain
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Submitted Jul 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Sox2 overexpressing Schwann cell)
Loading amount 25 µg
Specification Sox2 overexpressing Schwann cell
Gel Running Conditions Reduced Denaturing (12% SDS Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"