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Recombinant full length SOX2 protein (Human).
Our Abpromise guarantee covers the use of ab75485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC - Wholemount||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EPR3131] to SOX2 (ab92494). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Detection.|
|WB||1/200 - 1/2000. Predicted molecular weight: 34 kDa.|
|IHC-P||1/50 - 1/100.|
Immunocytochemistical detection of SOX2 antibody (ab75485) on a mixed rat glial cell culture. Fixative: Paraformaldehyde Permeabilization: 0.3x Triton. Blocking step: 10% serum for 1 hour @ 24°C. Primary antibody used at 1 µg/ml incubated for 24 hours @ 4°C. Secondary antibody: Alexa Fluor® 568 (1/1000). The submitted image shows rat primary glial culture stained with ab75485; DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"