Overview

  • Product name
  • Description
    Goat polyclonal to SOX2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Apteronotus leptorhynchus
  • Immunogen

    Synthetic peptide from Human SOX2 (UniProt ID: P48431).

  • Positive control
    • Human brain (cerebellum) and testis tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab110145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease
    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody images

  • ab110145, at 3.75 µg/ml, staining SOX2 in formalin fixed, paraffin embedded Human brain (cerebellum) by Immunohistochemistry.
  • ab110145, at 3.75 µg/ml, staining SOX2 in formalin fixed, paraffin embedded Human testis by Immunohistochemistry.

References for Anti-SOX2 antibody (ab110145)

This product has been referenced in:
  • Pakan JM & McDermott KW A method to investigate radial glia cell behavior using two-photon time-lapse microscopy in an ex vivo model of spinal cord development. Front Neuroanat 8:22 (2014). IHC (PFA fixed) . Read more (PubMed: 24782718) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (human tumor in mouse brain)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.2%
Specification
human tumor in mouse brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

Submitted Apr 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Blastocyst (D7 after IVF))
Specification
Blastocyst (D7 after IVF)
Fixative
Formaldehyde
Permeabilization
Yes - 1% Triton X-100 (30 min)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 12 2012

Vielen Dank fürIhre Anfrage.

Leider haben wirH00006657-A01 derzeit nicht in unserem Katalog verfügbar. Allerdings kann ich Ihnen gerne drei unserer anti-SOX2- Antikörper empfehlen, die wir für dieImmunhistochemie an humane...

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