Anti-SOX2 antibody (ab75627)


  • Product nameAnti-SOX2 antibody
    See all SOX2 primary antibodies
  • Description
    Rabbit polyclonal to SOX2
  • Tested applicationsSuitable for: ICC, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length SOX2 protein (Human)



Our Abpromise guarantee covers the use of ab75627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/1000.
    WB: 1/50 - 1/100. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
    • Sequence similaritiesContains 1 HMG box DNA-binding domain.
    • Post-translational
      Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANOP3 antibody
      • cb236 antibody
      • Delta EF2a antibody
      • lcc antibody
      • MCOPS3 antibody
      • MGC148683 antibody
      • MGC2413 antibody
      • RGD1565646 antibody
      • Sex determining region Y box 2 antibody
      • SOX 2 antibody
      • Sox2 antibody
      • SOX2_HUMAN antibody
      • SRY (sex determining region Y) box 2 antibody
      • SRY box containing gene 2 antibody
      • SRY related HMG box 2 antibody
      • SRY related HMG box gene 2 antibody
      • SRY-box 2 antibody
      • Transcription factor SOX 2 antibody
      • Transcription factor SOX-2 antibody
      • ysb antibody
      see all

    Anti-SOX2 antibody images

    • All lanes : Anti-SOX2 antibody (ab75627) at 1/50 dilution

      Lane 1 : 293 cell lysates nontransfected
      Lane 2 : 293 cell lysates transiently transfected with the SOX2 gene

      Lysates/proteins at 2 µg per lane.

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • ab75627 staining SOX2 from human neural stem cells derived from hESCscells by immunocytochemistry. Cells were paraformaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 18 hours at 4°C. An Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody. Left image shows nuclei staining with Dapi (blue) and right shows ab75627 staining (green).

      See Abreview

    References for Anti-SOX2 antibody (ab75627)

    ab75627 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply. I am very much in agreement with John that it would be difficult to purify ab92494 to such high concentrations but you should have no troubles in getting the 0.25mg/ml up to the levels you need. You needn't worry about our ...

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    Thank you for contacting us. John is away for a few weeks, my name is Keith.

    While I do not know exactly what you and John spoke of on the phone, from you email I have gathered that you are looking for very high affinity Sox2 antibodies for ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (Neural stem cells derived from hESCs)
    Specification Neural stem cells derived from hESCs
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Mr. Parinya Noisa

    Verified customer

    Submitted Oct 28 2009