Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179)

Overview

  • Product nameAnti-SOX2 antibody - Embryonic Stem Cell Marker
    See all SOX2 primary antibodies
  • Description
    Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 SOX2.

    (Peptide available as ab15831.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates; MEF1, MEL-1, F9, Mouse EG (TMAS), E14Tg2a, Hues7, 46C.
  • General notesab75179 is raised using the same immunogen as ab15830. Batches sold under ab75179 have passed QC testing in Western Blot only. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).Can be blocked with Human SOX2 peptide (ab15831).

Target

  • FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody - Embryonic Stem Cell Marker images

  • All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml

    Lane 1 : Hela Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
    Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179)

This product has been referenced in:
  • Cox JL  et al. Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells. J Cell Sci 124:2654-65 (2011). WB ; Human, Mouse . Read more (PubMed: 21750191) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Leucoraja erinacea Embryo (Whole embryo)
Specification Whole embryo
Username

Dr. Andrew Gillis

Verified customer

Submitted Jul 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryonic brain sections (E10.5))
Specification mouse embryonic brain sections (E10.5)
Fixative 4% PFA
Permeabilization Yes - 0.05% PBS-T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. veronique marthiens

Verified customer

Submitted Aug 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"