The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Antigen Retrieval is recommended, boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.
1/100. Predicted molecular weight: 34 kDa.
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Contains 1 HMG box DNA-binding domain.
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.