Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab24604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IHC-Fr.
  • Target

    Anti-SOX21 antibody images

    • Immunohistochemistry (Formaldehyde/PFA-fixed paraffin-embedded sections) using SOX21 antibody (ab24604)on Rat Tissue sections (adult brain lateral ventricle subventricular zone). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT°C. ab24604 used at 1/300 incubated for 16 hours. Secondary Antibody: biotin conjugated anti rabbit IgG (1/300). NB: Sox21 protein expression showin in this image is in concurrs with published mRNA positivity in the subventricular zone and rostro-migratory stream in the adult rat.

    References for Anti-SOX21 antibody (ab24604)

    ab24604 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (embryonic brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Sodium-citrate buffer, pH 6.0
    Permeabilization
    Yes - 0.3% Triton X-100 in PBS for 10 minutes
    Specification
    embryonic brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Laura Lahti

    Verified customer

    Submitted Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (adult brain lateral ventricle svz)
    Specification
    adult brain lateral ventricle svz
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 05 2007

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E12.5 Whole brain section)
    Specification
    E12.5 Whole brain section
    Fixative
    Paraformaldehyde
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Dr. Dean Griffiths

    Verified customer

    Submitted Aug 21 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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