Overview

  • Product name
  • Description
    Goat polyclonal to SOX3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide (

    C-DAASPLPGGRLHGVH

    ) corresponding to C terminal amino acids 413-427 of Human SOX3 (NP_005625.2).

  • Positive control
    • Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab104248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
  • Involvement in disease
    Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
    Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
    Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GHDX antibody
    • MRGH antibody
    • PHP antibody
    • PHPX antibody
    • sox3 antibody
    • SOX3_HUMAN antibody
    • SOXB antibody
    • SRY (sex determining region Y)-box 3 antibody
    • SRY Box 3 antibody
    • SRY-related HMG-box gene 3 antibody
    • Transcription factor Sox-3 antibody
    see all

Anti-SOX3 antibody images

  • Anti-SOX3 antibody (ab104248) at 0.5 µg/ml + Human Cerebral Cortex lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : ~42 kDa (why is the actual band size different from the predicted?)
  • ab104248 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows nuclear staining at verious intensities along the neuronal cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-SOX3 antibody (ab104248)

ab104248 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC+TRE-hSOX3)
Permeabilization
Yes - 0.1% PBS Triton
Specification
hESC+TRE-hSOX3
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 21 2017

Application
IHC - Wholemount
Sample
Mouse Embryo (E7.5 distal)
Specification
E7.5 distal
Username

Abcam user community

Verified customer

Submitted Jan 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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