• Product name
  • Description
    Rabbit polyclonal to SOX3
  • Host species
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Chicken, Zebrafish
  • Immunogen

    Synthetic peptide within Human SOX3 aa 80-129 (N terminal). The exact sequence is proprietary.


    Database link: P41225

  • Positive control
    • Raji cell lysate



Our Abpromise guarantee covers the use of ab42471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
  • Involvement in disease
    Defects in SOX3 are a cause of panhypopituitarism X-linked (PHPX) [MIM:312000]. Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary.
    Defects in SOX3 are the cause of mental retardation X-linked with isolated growth hormone deficiency (MRXGH) [MIM:300123].
    Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3) [MIM:300833]. A condition in which male gonads develop in a genetic female (female to male sex reversal). Note=Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • GHDX antibody
    • MRGH antibody
    • PHP antibody
    • PHPX antibody
    • sox3 antibody
    • SOX3_HUMAN antibody
    • SOXB antibody
    • SRY (sex determining region Y)-box 3 antibody
    • SRY Box 3 antibody
    • SRY-related HMG-box gene 3 antibody
    • Transcription factor Sox-3 antibody
    see all


  • Anti-SOX3 antibody (ab42471) at 0.5 µg/ml + Raji cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

    Diluted in 5% skim milk / PBS buffer, gel concentration 12%
  • Imunocytochemistry on primary murine neural stem cell cultures

    Cells were washed with PBS once and fixed in 4%PFA/PBS for 10 min at RT.
    Cells were permeabilized with 0.2% trition X-100/PBS (1ml/each well) for 20 min at RT
    Cells were blocked using 10%BSA/0.1% trition X-100/PBS (1ml/each well) for 30 min at RT
    Ab42471 was added at 1:500 and incubated over night at 4?.
    Cells were washed twice with 0.1% trition X-100/PBS.
    Cells were incubated with secondary Ab (Flourescein anti-mouse) at RT for 1 hour in the dark (1:200), followed by being washed with 0.1% trition X-100/PBS twice
    A: DAPI staining of the nuclei
    B: SOX3 staining using Ab42471
    Image submitted courtesy of: Shih-Han Lee
  • Western blot procedure primary murine neural stem cell cultures

    Proteins denaturized at 95oC for 10 min Run on 11% PAGE, and then transferred overnight to a nitrocellulose membrane
    Blocked in 5% non fat dried milk in PBST, 1 hr, RT shaking
    Ab42471 1:2000 dilution in 5% Marvel in PBST) , 1 hr, RT shaking
    Wash 3 time in PBST 10 min, RT shaking
    HRP anti-Rabbit (1:5000 dilution in 5% Milk in PBST),1 hr, RT shaking
    Wash 3 times in PBST 10 min, RT shaking
    incubate in ECL-Plus and exposed to film
    Data submitted courtesy of: Yu-Huan Shih


This product has been referenced in:
  • Corsinotti A  et al. Distinct SoxB1 networks are required for naïve and primed pluripotency. Elife 6:N/A (2017). Read more (PubMed: 29256862) »
  • Topalovic V  et al. Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. PLoS One 12:e0184099 (2017). Read more (PubMed: 28886103) »

See all 3 Publications for this product

Customer reviews and Q&As

IHC - Wholemount
Mouse Embryo (E7.5)

Abcam user community

Verified customer

Submitted Dec 05 2016

Thank you for your email. Since the peptide sequence is proprietary, we do not have this information about the peptide sequence used to generate this antibody. We can provide you with a 50 amino acid region from which the sequence was derived. This ...

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