Anti-SOX4 antibody [154C4a] (ab70598)
Key features and details
- Mouse monoclonal [154C4a] to SOX4
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-SOX4 antibody [154C4a]
See all SOX4 primary antibodies -
Description
Mouse monoclonal [154C4a] to SOX4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment of human SOX4 from an internal sequence.
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Positive control
- Recombinant human SOX4.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab70598 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
154C4a -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif. -
Tissue specificity
Testis, brain, and heart. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Ecotropic viral integration site 16 antibody
- EVI 16 antibody
- EVI16 antibody
see all
Images
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Anti-SOX4 antibody [154C4a] (ab70598) + immunising recombinant protein
Predicted band size: 47 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (12)
ab70598 has been referenced in 12 publications.
- Wu J et al. Circular RNA drives resistance to anti-PD-1 immunotherapy by regulating the miR-30a-5p/SOX4 axis in non-small cell lung cancer. Cancer Drug Resist 5:261-270 (2022). PubMed: 35800365
- Shen H et al. SOX4 promotes beige adipocyte-mediated adaptive thermogenesis by facilitating PRDM16-PPARγ complex. Theranostics 12:7699-7716 (2022). PubMed: 36451857
- Wu Y et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int 21:284 (2021). PubMed: 34051810
- Xie J et al. HLA complex group 11 is involved in colorectal carcinoma cisplatin resistance via the miR-214-5p/SOX4 axis. Oncol Lett 22:535 (2021). PubMed: 34079592
- Wang L et al. A novel tumour suppressor protein encoded by circMAPK14 inhibits progression and metastasis of colorectal cancer by competitively binding to MKK6. Clin Transl Med 11:e613 (2021). PubMed: 34709743