Anti-SOX4 antibody [154C4a] - ChIP Grade (ab70598)

Overview

  • Product nameAnti-SOX4 antibody [154C4a] - ChIP Grade
    See all SOX4 primary antibodies
  • Description
    Mouse monoclonal [154C4a] to SOX4 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant Fragment
  • Immunogen

    Recombinant fragment of human SOX4 from an internal sequence.

  • Positive control
    • Recombinant human SOX4.

Properties

Applications

Our Abpromise guarantee covers the use of ab70598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21878674
WB Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • FunctionTranscriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
  • Tissue specificityTestis, brain, and heart.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ecotropic viral integration site 16 antibody
    • EVI 16 antibody
    • EVI16 antibody
    • Sox4 antibody
    • SOX4_HUMAN antibody
    • SRY (sex determining region Y) box 4 antibody
    • SRY box containing gene 4 antibody
    • SRY related HMG box gene 4 antibody
    • Transcription factor SOX-4 antibody
    see all

Anti-SOX4 antibody [154C4a] - ChIP Grade images

References for Anti-SOX4 antibody [154C4a] - ChIP Grade (ab70598)

This product has been referenced in:
  • Lee H  et al. TMEM2 Is a SOX4-Regulated Gene That Mediates Metastatic Migration and Invasion in Breast Cancer. Cancer Res 76:4994-5005 (2016). Read more (PubMed: 27328729) »
  • Aue G  et al. Sox4 cooperates with PU.1 haploinsufficiency in murine myeloid leukemia. Blood 118:4674-81 (2011). ChIP ; Mouse . Read more (PubMed: 21878674) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (human normal keratinocytes and SCC cancer cell lin)
Specification human normal keratinocytes and SCC cancer cell lin
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2014

Thank you for your enquiry. I am pleased to hear this customer would like to make use of our testing discount offer and test ab70598SOX4 antibody [154C4a] in human samples.

I can provide the following code:

DISCOUNT CODE: #### Read More

Thank you for your phone call. I am sorry to hear that ab80261 did not work as expected. Unfortunately, since the antibody was purchased over 1 year ago and our guarantee is valid for 6 months after the purchase, I am not able to offer a free of cha...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (cancer cells)
Loading amount 30 µg
Specification cancer cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"