Our Abpromise guarantee covers the use of ab26041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/500. PubMed: 19521496|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
ab26041 at 1/500 dilution staining SOX5 in chicken embryo head section by immunohistochemistry (Frozen sections). Sections were incubated overnight in 30% sucrose in PBS at 4°C, embedded in OCT freezing medium, frozen and sectioned in a cryostat. After fixation sections were rehydrated in PBS for 15 min and then blocked in PBS containing 1% fetal calf serum, 0.1% Triton-X and 0.02% Thimerosal. Primary antibody was used overnight at 4°C. The secondary antibody was used at 1/200 dilution for 2 hours.