This antibody has been tested in IHC-P in wildtype post-natal mouse brain sections and showed specific staining. No immunoreactivity was observed in control samples of post-natal brain from a Sox6 knockout mouse.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody - ChIP Grade (ab30455)This image is courtesy of Eiman Azim, Deb Schuback and Jeff Macklis, Massachusetts General Hospital - Harvard Medical School Center for Nervous System Repair
ab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX6 antibody - ChIP Grade (ab30455)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, France
IHC-FoFr image of Sox6 staining in Rat hippocampus sections using ab30455.The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. immunostainings were performed using the ‘free floating’ technique.
Stelcer E et al. Expression of Pluripotency Genes in Chondrocyte-Like Cells Differentiated from Human Induced Pluripotent Stem Cells. Int J Mol Sci19:N/A (2018).
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