• Product name
  • Description
    Rabbit polyclonal to SOX6
  • Specificity
    This antibody detects endogenous levels of total SOX6 protein.
  • Tested applications
    Suitable for: ChIP/Chip, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) from the N terminus of human SOX6.

  • Positive control
    • Jurkat cell extract



Our Abpromise guarantee covers the use of ab64946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP/Chip Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration.


  • Function
    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • Tissue specificity
    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    Sumoylation inhibits the transcriptional activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all


  • All lanes : Anti-SOX6 antibody (ab64946) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa


This product has been referenced in:
  • Jia Z  et al. SOX6 and PDCD4 enhance cardiomyocyte apoptosis through LPS-induced miR-499 inhibition. Apoptosis 21:174-83 (2016). WB . Read more (PubMed: 26659076) »
  • Mitchell J  et al. ß2-adrenergic receptors inhibit the expression of collagen type II in growth plate chondrocytes by stimulating the AP-1 factor Jun-B. Am J Physiol Endocrinol Metab 300:E633-9 (2011). WB ; Mouse . Read more (PubMed: 21177286) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry.
Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.
We aim to provide as much information as possible to our customers, so I am sorry that...

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Thank you for your enquiry regarding ab64946 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applie...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet...

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Thank you for contacting Abcam.
The antibody should be completely fine with being stored at 4C for a week, you should not see an adverse affects with the performance of the antibody. If the antibody was stored for longer at 4C rather than -20C, th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Lung, A549 cell line)
Lung, A549 cell line
Yes - TRITON .25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 09 2012

Thank you very much for your interest in our SOX6 antibodies.

I am happy to let you know that we have an anti-SOX6 antibody available that is guaranteed to work in ChIP with human, mouse or rat samples:
ab64946 : Click here (or use the ...

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Thank you for confirming the order information. I have processed the customer’s request to receive ab33875 as a replacement antibody for ab64649 which did not work in western blot. If there is anything else I can help you with, please let me know.

Thank you for your reply. I would be happy to send ab33875 as a replacement. I think I have found the original order but I would just like to confirm before placing the replacement request. Was the order number ****** and was it placed on the 29th June...

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Thank you for your reply. I am sorry about the confusion over the block, I misread your notes that you sent, as I thought that you were blocking in 5% Milk and incubating the primary in BSA and I thought that by blocking and incubating in the same buff...

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Thank you for contacting Abcam. I am sorry that you are experiencing trouble with ab64946 in western blot. From the information and images you provided I have a few suggestions that may help reduce background and increase specificity. 1 - I would recom...

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1-10 of 14 Abreviews or Q&A


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