This image shows notochord of 12 d.p.c. mouse embryos stained with ab66316 at 1/100 dilution. Detection was done using anti-rabbit-Cy3 conjugated secondary antibodies (red fluorescence). Cell nuceli were counterstained with DAPI (blue fluorescence).
Western blot - Anti-SOX6 antibody (ab66316)
All lanes : Anti-SOX6 antibody (ab66316) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa Observed band size : 93 kDa Additional bands at : 20 kDa,50 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Wang X et al. Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo. Development138:4399-404 (2011).
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