Overview

  • Product nameAnti-SOX6 antibody
    See all SOX6 primary antibodies
  • Description
    Rabbit polyclonal to SOX6
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Non Human Primates
  • Immunogen

    Synthetic peptide:

    YDDYEDDPKSDYSSENE

    , corresponding to amino acids 780-796 of Human SOX6

  • Positive control
    • This antibody gave a positive signal in HeLa and Jurkat Nuclear Extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab66316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21880783

Target

  • FunctionTranscriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • Tissue specificityExpressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits the transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Anti-SOX6 antibody images

  • This image shows notochord of 12 d.p.c. mouse embryos stained with ab66316 at 1/100 dilution. Detection was done using anti-rabbit-Cy3 conjugated secondary antibodies (red fluorescence). Cell nuceli were counterstained with DAPI (blue fluorescence).
  • All lanes : Anti-SOX6 antibody (ab66316) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 20 kDa,50 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds

References for Anti-SOX6 antibody (ab66316)

This product has been referenced in:
  • Wang X  et al. Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo. Development 138:4399-404 (2011). IHC-Fr ; Zebrafish . Read more (PubMed: 21880783) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"