Overview

  • Product name
  • Description
    Rabbit polyclonal to SOX7
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: MASLLGAYPW TEGLECPALE AELSDGLSPP AVPRPSGDKS SESRIRRPMN, corresponding to N terminal amino acids 1-50 of Mouse SOX7

  • Positive control
    • SP2/0 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-SOX7 antibody images

  • Anti-SOX7 antibody (ab49163) at 1.25 µg/ml + SP2/0 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000 dilution

    Predicted band size : 41 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SOX7 antibody (ab49163) at 2 µg/ml

    Lane 1 : Lung lysate
    Lane 2 : Heart lysate
    Lane 3 : Liver lysate
    Lane 4 : Negative control - Kidney lysate
    Lane 5 : Negative control- 293T lysate
    Lane 6 : Negative control - HepG2 lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Rabbit IgG Antibody - HRP Conjugated

    Predicted band size : 41 kDa

    Blocking buffer: 3% milk 0.05% TBST

References for Anti-SOX7 antibody (ab49163)

ab49163 has not yet been referenced specifically in any publications.

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