Overview

  • Product nameAnti-SOX7 antibody
    See all SOX7 primary antibodies
  • Description
    Rabbit polyclonal to SOX7
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Pufferfish (Fugu), Zebrafish
  • Immunogen

    Synthetic peptide: PGDKGSESRI RRPMNAFMVW AKDERKRLAV QNPDLHNAEL SKMLGKSWKA , corresponding to N terminal amino acids 37-86 of Human SOX7

  • Positive control
    • 293T nuclear lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-SOX7 antibody images

  • Anti-SOX7 antibody (ab80331) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling SOX7 with ab80331 at 5.0µg/ml.

References for Anti-SOX7 antibody (ab80331)

This product has been referenced in:
  • Wu GG  et al. Mir-184 post-transcriptionally regulates SOX7 expression and promotes cell proliferation in human hepatocellular carcinoma. PLoS One 9:e88796 (2014). WB ; Human . Read more (PubMed: 24558429) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"