Overview

  • Product nameAnti-SOX8 antibody
    See all SOX8 primary antibodies
  • Description
    Rabbit polyclonal to SOX8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX8.

  • Positive control
    • This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).

Target

Anti-SOX8 antibody images

  • All lanes : Anti-SOX8 antibody (ab104245) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

References for Anti-SOX8 antibody (ab104245)

ab104245 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ChIP
Sample Human Cell lysate - nuclear (Sarcoma cancer cells expressing SOX8)
Negative control Heterochromatic gene desert
Specification Sarcoma cancer cells expressing SOX8
Detection step Semiquantitative PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 12 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde in DMEM
Positive control Active Super Enhancer site
Username

Berkley Gryder

Verified customer

Submitted Dec 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"