Overview

  • Product nameAnti-SOX8 antibody
    See all SOX8 primary antibodies
  • Description
    Rabbit polyclonal to SOX8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human SOX8.

  • Positive control
    • This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionMay play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC24837 antibody
    • SOX 8 antibody
    • Sox8 antibody
    • SOX8_HUMAN antibody
    • SRY (sex determining region Y) box 8 antibody
    • SRY box 8 antibody
    • SRY box containing gene 8 antibody
    • Transcription factor SOX 8 antibody
    • Transcription factor SOX-8 antibody
    • Transcription factor SOX8 antibody
    see all

Anti-SOX8 antibody images

  • All lanes : Anti-SOX8 antibody (ab125858) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,255 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • All lanes : Anti-SOX8 antibody (ab125858) at 1/700 dilution

    Lane 1 : Glioblastoma cell line expressing high Sox8 according to RealTime PCR data.
    Lane 2 : Glioblastoma cell line expressing low Sox8 according to RealTime PCR data.
    Lane 3 : Glioblastoma cell line expressing low Sox8 according to RealTime PCR data.

    Secondary
    Standard HRP secondary at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image was taken from an anonymous abreview.

    See Abreview

References for Anti-SOX8 antibody (ab125858)

ab125858 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma cell lines)
Loading amount 5 µg
Specification Glioblastoma cell lines
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
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Submitted Mar 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"