Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag
Our Abpromise guarantee covers the use of ab76997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.|
|ELISA||Use at an assay dependent concentration. Detection limit for ab76997 is approximately 30 ng/ml when used as a capture antibody.|
|IHC-P||Use a concentration of 0.7 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|