Overview

  • Product nameAnti-SOX9 antibody [3C10]
    See all SOX9 primary antibodies
  • Description
    Mouse monoclonal [3C10] to SOX9
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag

  • Positive control
    • Immunogen, HepG2 cell lysate and human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab76997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent concentration. Detection limit for ab76997 is approximately 30 ng/ml when used as a capture antibody.
IHC-P Use a concentration of 0.7 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 antibody [3C10] images

  • Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 56 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Immunoperoxidase analysis of SOX9 expression on formalin fixed, paraffin embedded human tonsil tissue using 0.7 µg/ml of ab76997.
  • Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.
  • Overlay histogram showing HepG2 cells stained with ab76997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76997, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-SOX9 antibody [3C10] (ab76997)

This product has been referenced in:

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Normal Goat Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Tissue sections (Spinal Cord)
Specification Spinal Cord
Permeabilization No
Fixative Paraformaldehyde
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Submitted Oct 27 2014

I have processed your request to receive ab118892 as a replacement for ab76997. Your new order number is ******, and the antibody will ship out as soon as it comes into stock.

Application Immunocytochemistry
Sample Mouse Cultured Cells (cortical neurons)
Specification cortical neurons
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
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Submitted Jun 06 2016

Application Immunocytochemistry
Sample Human Cultured Cells (neural precourser cell derived from iPSC)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification neural precourser cell derived from iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Apr 27 2016

Application IHC - Wholemount
Sample Human Tissue (Liver)
Specification Liver
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Submitted Nov 16 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (muller Cell, Retina)
Specification muller Cell, Retina
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted Aug 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (PSYPPITRSQ peptide)
Specification PSYPPITRSQ peptide
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Type Direct
Username

Abcam user community

Verified customer

Submitted Mar 14 2013

Thank you for contacting us.

We can reassure you that the antibody is SOX9 specific, it should not be cross reacting with SOX10.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

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Thank you very much for your interest in ab76997

To our knowledge, ab/xxx/ has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab76997 now, test it in Flow Cyto and submit feedback to us in...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Username

Mr. Benny Garfinkel

Verified customer

Submitted Jun 22 2012

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"