SOX9 antibody (ab3697)

Product Name 

SOX9 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SOX9

Immunogen 

Synthetic peptide: KYQPRRRKSVKNG, corresponding to amino acids 173 - 185 of Human / Mouse / Bovine SOX 9.
BLAST 'KYQPRRRKSVKNG' with or

Reacts with 

Ms, Rat

Predicted to react 
(species key)

Hu, Cow
(Due to sequence homology)

Specificity 

The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared SOX9 peptides. By immunoblot analysis, a ~65 kDa immunoreactive protein from mouse embryo cell lysate was detected (sample were boiled prior to subject 10% SDS-polyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic peptide.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1 µg/ml.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: 1/200 (PMID 19388005).
IP: Use 3-5µg for 10⁷ cells.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 56 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

3T3 cells

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis

Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box

Relevance 

SOX9 is a member of the family of SOX (Sry-type high mobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for SOX9 antibody images, references, products related to ab3697 and other tools.

SOX9 antibody images:

References for SOX9 antibody (ab3697)

  This product has been used in: (two most recent references)

El-Helou V et al. The cardiac neural stem cell phenotype is compromised in streptozotocin-induced diabetic cardiomyopathy. J Cell Physiol 220:440-9 (2009). WB, IHC-Fr; Rat. PubMed: 19388005

Passeron T et al. SOX9 is a key player in ultraviolet B-induced melanocyte differentiation and pigmentation. Proc Natl Acad Sci U S A 104:13984-9 (2007). PubMed: 17702866

See all 4 publication references for this product.


If you publish research using ab3697 please let us know so that we can cite the reference on this datasheet.

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