Overview

  • Product nameAnti-SOX9 antibody
    See all SOX9 primary antibodies
  • Description
    Rabbit polyclonal to SOX9
  • Specificityab71762 recognizes human SOX9 protein without phosphorylation at S181. It does not recognize SOX9 protein phosphorylated at S181.
  • Tested applicationsSuitable for: EMSA, IHC-P, Dot blot, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic non-phosphopeptide (Human) surrounding the epitope RKSVK (amino acids 179 - 183) from SOX9.

  • Positive control
    • LNCaP prostate Carcinoma whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Dot blot 1/1000.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Target

  • FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 antibody images

  • All lanes : Anti-SOX9 antibody (ab71762) at 1/500 dilution

    Lane 1 : LNCaP prostate Carcinoma whole cell lysate
    Lane 2 : Negative control


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab71762 at 1/1000 dilution staining 1 µg of the following peptides on nitrocellulose membrane:
    A: SOX9 (pS181)
    B: SOX9 (Paired S181) (Nonphospho)
    C: Non-related phosphospecific PP.
  • Immunohistochemical analysis of Eublepharis macularius tail tissue, staining SOX9 with ab71762.

    Sections were blocked with 3% normal goat serum in PBS for 1 hour at room temperature and incubated overnight at 4°C with primary antibody (1/600 in PBS). Staining was detected with DAB.

References for Anti-SOX9 antibody (ab71762)

This product has been referenced in:
  • McLean KE & Vickaryous MK A novel amniote model of epimorphic regeneration: the leopard gecko, Eublepharis macularius. BMC Dev Biol 11:50 (2011). IHC-P . Read more (PubMed: 21846350) »
  • Betancur P  et al. Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest. Proc Natl Acad Sci U S A 107:3570-5 (2010). EMSA . Read more (PubMed: 20139305) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (duodenum)
Specification duodenum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Bond Epitope Retrieval 2
Permeabilization No
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Submitted May 14 2013

Gracias por contactarnos.

Varios marcadores pueden usarse para la detección de condrocitos mediante immunohistoquímica. Solo algunos de ellos se han testado en especie canina, el resto me temo que no se han testado, por lo que s...

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