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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-SOX9 antibody - ChIP Grade
See all SOX9 products (13) ...
Description
Rabbit polyclonal to SOX9 - ChIP Grade
Specificity
The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared SOX9 peptides. By immunoblot analysis, a ~65 kDa immunoreactive protein from mouse embryo cell lysate was detected (sample were boiled prior to subject 10% SDS-polyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic peptide.
Tested applications
IHC-Fr, ICC/IF, WB, IP, ELISA, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Predicted to work with
Cow
Immunogen
Synthetic peptide: KYQPRRRKSVKNG, corresponding to amino acids 173 - 185 of Human / Mouse / Bovine SOX 9.
KYQPRRRKSV KNG
Positive control
3T3 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
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Western blot - Anti-SOX9 antibody (ab3697)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/200(PubMed: 19388005)
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 62 kDa (predicted molecular weight: 56 kDa).
IP: Use 3-5µg for 107 cells.
ELISA: Use a concentration of 0.1 - 1 µg/ml.
ChIP: Use at an assay dependent concentration. (PubMed: 21872590)
Target
Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Database links
- Entrez Gene: 374148 Chicken
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: P48434 Chicken
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
see all
Anti-SOX9 antibody - ChIP Grade images:
Western blot - Anti-SOX9 antibody (ab3697)
Predicted band size : 56 kDa
Rabbit polyclonal antibody to Sox9 recognizes full length of Sox9 (left lane) and C-terminal deletion of Sox9 (right lane)
(Compared to anti-Sox9 antibody from a different company - not shown).
This Western blot image was kindly supplied as part of the review submitted by Lilia Topol.
References for Anti-SOX9 antibody - ChIP Grade (ab3697)
This product has been referenced in:
- Shen Jet al. Arterial injury promotes medial chondrogenesis in Sm22 knockout mice. Cardiovasc Res 90:28-37 (2011). IHC-Fr; Mouse.Read more (PubMed: 21183509) »
- Aza-Carmona Met al. SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. Hum Mol Genet 20:1547-59 (2011).Read more (PubMed: 21262861) »
See all 10 publications for this product
Publishing research using ab3697? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"