Anti-SOX9 (phospho S181) antibody (ab68610)

Overview

  • Product nameAnti-SOX9 (phospho S181) antibody
    See all SOX9 primary antibodies
  • Description
    Rabbit polyclonal to SOX9 (phospho S181)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog, Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human SOX9, phosphorylated at S181 and phosphorylated at S181.

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human SOX9, phosphorylated at S181 and phosphorylated at S181.

  • Positive control
    • This antibody gave a positive signal in Human bone marrow and liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab68610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 (phospho S181) antibody images

  • All lanes : Anti-SOX9 (phospho S181) antibody (ab68610) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Modified blocking peptide at 1 µg/ml
    Lane 4 : Human liver tissue lysate - total protein (ab29889) with Modified blocking peptide at 1 µg/ml
    Lane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Non - Modified blocking peptide at 1 µg/ml
    Lane 6 : Human liver tissue lysate - total protein (ab29889) with Non - Modified blocking peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 15 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-SOX9 (phospho S181) antibody (ab68610)

ab68610 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample (Paddlefish) Polyodon spathula Tissue sections (cartilage)
Specification cartilage
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"