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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SP100.
(Peptide available as ab44037.)
Our Abpromise guarantee covers the use of ab43151 in the following tested applications.
ab43151 staining SP100 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with peroxidase for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody.