Overview

  • Product nameAnti-SP100 antibody
    See all SP100 primary antibodies
  • Description
    Mouse monoclonal to SP100
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MAGGGGDLST RRLNECISPV ANEMNHLPAH SHDLQRMFTE DQGVDDRLLY DIVFKHFKRN KVEISNAIKK TFPFLEGLRD RDLITNKMFE DSQDSCRN, corresponding to amino acids 1-99 of Human SP100

Properties

Applications

Our Abpromise guarantee covers the use of ab58206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in the control of gene expression.
    • Tissue specificityWidely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.
    • Sequence similaritiesContains 2 HMG box DNA-binding domains.
      Contains 1 HSR domain.
      Contains 1 SAND domain.
    • DomainThe HSR domain is important for the nuclear body targeting as well as for the dimerization.
      Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • Post-translational
      modifications
      Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.
    • Cellular localizationNucleus > PML body. Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686E07254 antibody
      • FLJ00340 antibody
      • FLJ34579 antibody
      • Lysp100b antibody
      • Nuclear antigen Sp100 antibody
      • Nuclear autoantigen Sp 100 antibody
      • Nuclear autoantigen Sp-100 antibody
      • Nuclear autoantigen Sp100 antibody
      • Nuclear dot associated Sp100 protein antibody
      • Nuclear dot-associated Sp100 protein antibody
      • SP 100 antibody
      • SP100 antibody
      • SP100 HMG nuclear autoantigen antibody
      • SP100 nuclear antigen antibody
      • SP100_HUMAN antibody
      • Speckled 100 kDa antibody
      see all

    Anti-SP100 antibody images

    • Western blot against tagged recombinant protein immunogen using ab58206 SP100 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-SP100 antibody (ab58206)

    This product has been referenced in:
    • Schmeisser H  et al. Identification of alpha interferon-induced genes associated with antiviral activity in Daudi cells and characterization of IFIT3 as a novel antiviral gene. J Virol 84:10671-80 (2010). WB ; Human . Read more (PubMed: 20686046) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab58206.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"