Overview

  • Product nameAnti-SP140 antibody
    See all SP140 primary antibodies
  • Description
    Rabbit polyclonal to SP140
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Pig
  • Immunogen

    A region within a synthetic peptide: VCPEPIFRFF RENKVEIASA ITRPFPFLMG LRDRSFISEQ MYEHFQEAFR, corresponding to N terminal amino acids 37-86 of Human SP140

  • Positive control
    • Raji cell lysate for WB, and epithelial cells of renal tubule for IP.

Properties

Applications

Our Abpromise guarantee covers the use of ab24341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionComponent of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection.
  • Tissue specificityHigh levels in spleen and peripheral blood leukocytes, much lower levels in thymus, prostate, ovary, small intestine, and colon. Very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
  • Sequence similaritiesContains 1 bromo domain.
    Contains 1 HSR domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 SAND domain.
  • Cellular localizationNucleus. Cytoplasm. Localized to nuclear structures termed LANDS, for LYSp100-associated nuclear domains. LANDS are globular, electron-dense structures most often found in the nucleoplasm, but also found at the nuclear membrane and in the cytoplasm, suggesting that these structures may traffic between the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LY10_HUMAN antibody
    • Lymphoid restricted homolog of Sp100 antibody
    • Lymphoid specific SP100 homolog antibody
    • Lymphoid-restricted homolog of Sp100 antibody
    • LYSP100 A antibody
    • LYSp100 antibody
    • LYSP100 B antibody
    • LYSp100 protein antibody
    • LYSP100-A antibody
    • LYSP100-B antibody
    • LYSP100A antibody
    • LYSP100B antibody
    • MGC126440 antibody
    • Nuclear antigen Sp140 antibody
    • Nuclear autoantigen Sp-140 antibody
    • Nuclear autoantigen Sp140 antibody
    • Nuclear body protein SP140 antibody
    • SP140 antibody
    • SP140 nuclear body protein antibody
    • SP140 PEN antibody
    • Speckled 140 kDa antibody
    see all

Anti-SP140 antibody images

  • Anti-SP140 antibody (ab24341) at 5 µg/ml + Raji cell lysate.

    Secondary
    HRP conjugated rabbit anti SP140 IgG, at a dilution of 1/50000 - 1/100000.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SP140 with ab24341 at a concentration of 4-8ug/ml. Renal tubule epithelial cells stained positive are indicated by arrows. Magnification: X400.

     

     

References for Anti-SP140 antibody (ab24341)

ab24341 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am sorry for the delay to your enquiry. The sequence of this immunogen is proprietary. However, I can tell you that the antigenic peptide is derived from the first 50 aa residues of the N terminal region of SP140. I ho...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"