Overview

  • Product nameAnti-SP3 antibody
    See all SP3 primary antibodies
  • Description
    Rabbit polyclonal to SP3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C-terminus of human SP3.

  • Positive control
    • extracts from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab72594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
ELISA 1/10000.

Target

  • FunctionTranscriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Not glycosylated.
    Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
    Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
  • Cellular localizationNucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • D130027J01Rik antibody
    • DKFZp686O1631 antibody
    • GC binding transcription factor Sp 3 antibody
    • GC binding transcription factor Sp3 antibody
    • MGC105187 antibody
    • OTTMUSP00000014207 antibody
    • SP 3 antibody
    • Sp 3 transcription factor antibody
    • SP3 antibody
    • Sp3 transcription factor antibody
    • SP3_HUMAN antibody
    • Specificity protein 3 antibody
    • SPR 2 antibody
    • SPR-2 antibody
    • SPR2 antibody
    • Transcription factor SP 3 antibody
    • Transcription factor Sp3 antibody
    see all

Anti-SP3 antibody images

  • All lanes : Anti-SP3 antibody (ab72594) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells, with 10 ug of the immunising peptide.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 82 kDa

References for Anti-SP3 antibody (ab72594)

This product has been referenced in:
  • Bergeron F  et al. Transcription of platelet-derived growth factor receptor {alpha} in Leydig cells involves specificity protein 1 and 3. J Mol Endocrinol 46:125-38 (2011). Read more (PubMed: 21289081) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"