Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse Sp7/ Osterix.
(Peptide available as ab24390.)
Our Abpromise guarantee covers the use of ab22552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 20682789|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20682789|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. PubMed: 17510056|
|IHC-P||1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.|
|IP||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20410296|
ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.
Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.
ab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
Image courtesy of Human Protein Atlas
ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ab22552 staining Sp7 / Osterix in 21 days old rat developing long bone tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocking with 20% serum at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) for 3 hours at 220C. A Cy3®-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.