Anti-Sp7 / Osterix antibody - ChIP Grade (ab22552)

Overview

  • Product name
    Anti-Sp7 / Osterix antibody - ChIP Grade
    See all Sp7 / Osterix primary antibodies
  • Description
    Rabbit polyclonal to Sp7 / Osterix - ChIP Grade
  • Tested applications
    Suitable for: IHC-Fr, ChIP, ICC/IF, WB, IHC-P, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse Sp7/ Osterix.

    (Peptide available as ab24390.)

  • Positive control
    • This antibody gave a positive signal for Immunohistochemistry (FFPE) in Rat bone.

Properties

Applications

Our Abpromise guarantee covers the use of ab22552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
ChIP Use at an assay dependent concentration. PubMed: 20682789
ICC/IF Use at an assay dependent concentration. PubMed: 20682789
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. PubMed: 17510056
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20410296

Target

Anti-Sp7 / Osterix antibody - ChIP Grade images

  • ab22552 staining Sp7/ Osterix in C2C12 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were stained with ab22552 at a 1/500 dilution followed by goat anti-rabbit IgG conjugated with Alexa Fluor 488 at a 1/400 dilution. Labeling was detected using a Leica TCS SL inverted laser scanning confocal microscope.

    The subcellular localization of Osterx in C2C12 cells was analyzed by immunofluorescence with ab22552 in cells either transiently transfected with short Osterix construct or incubated in medium without serum. Scale bar 10 µm.
  • Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

  • ab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • Image courtesy of Human Protein Atlas

    ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

  • The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

    See Abreview

  • ab22552 staining Sp7 / Osterix in 21 days old rat developing long bone tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocking with 20% serum at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) for 3 hours at 220C. A Cy3®-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.

    See Abreview

References for Anti-Sp7 / Osterix antibody - ChIP Grade (ab22552)

This product has been referenced in:
  • Chen Y  et al. An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs). Sci Rep 7:44130 (2017). WB, IHC-P ; Rat . Read more (PubMed: 28272494) »
  • He G  et al. Differential involvement of Wnt signaling in Bmp regulation of cancellous versus periosteal bone growth. Bone Res 5:17016 (2017). Mouse . Read more (PubMed: 28607813) »

See all 80 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (calvaria bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris-Based Catalog Number: H-3301
Permeabilization
Yes - Triton100
Specification
calvaria bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Tina Yuan

Verified customer

Submitted Dec 19 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization
No
Specification
Bone
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted Apr 05 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone Marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization
No
Specification
Bone Marrow
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted Apr 05 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (periodontal ligament)
Specification
periodontal ligament
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 17 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% gel)
Sample
Rat Cell lysate - whole cell (Primary cultured osteoblasts)
Specification
Primary cultured osteoblasts
Blocking step
Membrane blocking agent (GE Healthcare) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

幸弘 小原

Verified customer

Submitted Jul 24 2014

Application
ChIP on chip
Sample
Mouse Cell lysate - whole cell (mc3t3-e1)
Specification
mc3t3-e1
Type
Native ChIP (N-ChIP)
Detection step
Microarray
Positive control
Osterix transfection in mc3t3-e1 cell line
Negative control
not treated
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse cell)
Total protein in input
500 µg
Specification
mouse cell
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

Thank you for contacting us.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products. However, I have passes on your details to our distributor (allied scientific). I am currently in contact ...

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I have checked this antibody and it is suitable for use in WB and IHC. It is unfortunate that you did not get satisfactory results and it appears that you may have received a faulty vial. I have forwarded your cas...

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I have had a look at our records, and have found one order (Order number: xxxxxxxxx) placed by our distributor Allied Scientific Products in India. According to this order, it was placed xxxxxxxxx which is 1 year ...

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